SKU: 76488806369

Canine IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit

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Description

Canine IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA KitThe Canine IGFBP 3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit measures IGFBP 3 in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with IGFBP 3. IGFBP 3 in the sample or standard competes with a fixed amount of biotin labeled IGFBP 3 for sites on a pre coated antibody specific to

The Canine IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit measures IGFBP-3 in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with IGFBP-3. IGFBP-3 in the sample or standard competes with a fixed amount of biotin-labeled IGFBP-3 for sites on a pre-coated antibody specific to IGFBP-3. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of IGFBP-3 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 76488806369

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4.6 ★★★★★
Based on 22 reviews
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M
Verified Purchase
M Weidner
Whiting, US
★★★★★ 3
Liked it
Format: Kindle
I liked the worlds and the characters. The prophesy is confusing in that I would think, since all worlds ore on a path to destruction, the angels would want their world to be saved. I am sure there is more evil that will be revealed as the series proceeds. I dislike a prolonged “poor little me” trope and the main character has moved past that, thankfully, and began to show a fierce attitude as the novel progressed. The last portion of the novel had some great action. It’s worth reading, just be patient with a slow start.
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Reviewed in the United States on February 14, 2024
J
Verified Purchase
Julie R.
Port Orchard, US
★★★★★ 5
Great start
Format: Kindle
I loved this book! It's funny but still deals with tough themes, like chronic illness, a serial killer on the loose, and a dash of self-harm. The guys are interesting and distinct, we don't know too much about them yet. It does end on a really terrible cliffhanger but on the bright side the next book is out and I believe the series is complete. I have enjoyed both of these authors separately and this is a great team up!
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Reviewed in the United States on May 2, 2025
S
Verified Purchase
S T
Charlottesville, US
★★★★★ 4
Fudge!
Format: Kindle
Titles aren’t my strong suit. Sorry not sorry. That ending has me completely frustrated book two isn’t out yet. Just throwing that out there. First thing first, this is a high school book where the lead is under 18. Yea yea she’s about month from being an adult, but I can’t say I would’ve even opened the file if I’d realized. It’s not a smut fest or anything, but there is sexual tension as each party figures out their emotions. That being said, it’s a good book. There are some troubling parts, like how the supes come into their powers at 13, but most of those are dealt with in a way that makes sense. Kian is the exception. His whole arc pisses me off, especially since no one stepped in. Reflecting back on real world situations, ten or so years ago, I can see it happening, but it still makes me sick. Which, I’m sure was the whole damn point. For the most part, Courting Darkness is a fun read. I found myself laughing along with most of the set up. By the time it started getting serious, I’d grown to like the characters enough it held an impact. I’ll be adding book two to my list for when it comes out.
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Reviewed in the United States on March 8, 2022
D
Verified Purchase
Dee
Port Orchard, US
★★★★★ 5
Intriguing
Format: Kindle
An intriguing story that played out slowly while introducing some unique characters. The story became quite fascinating to read as these same characters provided startling revelations to their lives. As their relationships evolve, more drama and mystery is revealed adding to my fascination. I can’t wait to read more.
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Reviewed in the United States on May 27, 2026
D
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Darcy Smith
Lake Worth, US
★★★★★ 5
Outstanding Story!!!!!!!
Format: Kindle
Oh I loved this story. Sera is such an amazing, fun character. She has so much fire inside of herself. She has humor that makes you laugh with her sassy comments. The guys are fantastic. I don’t feel as if we really have gotten to know who and what they are but they are such a mix of fae. The story is extremely well written and developed. It had me pulled in and didn’t let go. There was so much action, drama, and suspense. Then we get to the ending and goodness there is so much going on. This ending was a major cliffhanger and I absolutely can’t wait to see what happens next.
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Reviewed in the United States on March 12, 2024

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