SKU: 445458257

Human DHVD3 (1, 25-dihydroxyvitamin D3, DVD) ELISA Kit

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Description

Human DHVD3 (1, 25-dihydroxyvitamin D3, DVD) ELISA KitThe Human (DHVD3) 1, 25 dihydroxyvitamin D3, DVD ELISA Kit measures 1,25 dihydroxyvitamin D3 in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB

The Human (DHVD3) 1, 25-dihydroxyvitamin D3, DVD ELISA Kit measures 1,25-dihydroxyvitamin D3 in samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of DVD,DHVD3 in the sample. The concentration of DVD,DHVD3 in the sample is then determined by comparing the O.D. of the samples to the standard curve.

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SKU: 445458257

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4.8 ★★★★★
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Eric
Alexandria, US
★★★★★ 2
Good finish, poor hardware.
Set screws are poor quality and are sometimes powdercoated on the exposed threads. Leads to striped hex sockets and inability to tighten properly. Durable finish as evidenced by no visible damage after throwing parts across the shop in frustration. Ended up running self tapping screws thru parts to complete assembly.
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Reviewed in the United States on February 26, 2024
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Carl L. Bradley
Boise, US
★★★★★ 1
Kinda flimsy…
Thought the assembly was metal, it’s some kind of PVC.
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Reviewed in the United States on September 22, 2025
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DRJRRT
Dallas, US
★★★★★ 5
Great references book.
Format: Hardcover
Excellent book for medical reference and refresh of knowledge.
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Reviewed in the United States on April 28, 2026
R
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Ray Schaar
Carnegie, US
★★★★★ 5
A Wonderful Update To A Great Old Reference Book!
Format: Hardcover
It's the Merck Manual! If you know medicine but are aware you don't know EVERYTHING about EVERYTHING! (Few if any do!) This is a wonderful reference. If you have no medical knowledge and think this will make you know what doctors know. Don't waste your money. You have to have a basic understanding for this to be helpful. This is a wonderful update to an old favorite.
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Reviewed in the United States on July 16, 2025
M
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Majted
Louisville, US
★★★★★ 5
The most current and inclusive medical reference available mid-2018.
Format: Hardcover, Format: Hardcover
Here’s the review I wrote for the 19th edition on 2011: I've been getting and using the Merck Manual for over 30 years to answer questions about disease for family and friends (pediatrics through geriatrics) as well as preparing to ask germane questions of physicians responsible for care of family and friends. It is comprehensive in its coverage of the etiology, diagnosis, treatment and prognosis of diseases of the human body and contains the latest research results on the treatment options. It's really not that difficult for the layman to understand the medical terminology and the result is factual understanding of the major aspects of disease which you will NOT get from the standard home health care books from Johns Hopkins, Harvard, Mayo Clinic, the AMA or even the Merck Home Health handbook. And, unlike the Merck Manual, these books directed to the laity do not contain the diagnostic information upon which physicians decide precisely what disease they are presented with. Yes, there will be much superfluous information which you may never use, but, when you want and need the information about a specific disease, here's where you should start your education. The same holds true for the updated 2018 20th edition. I neglected to mention that this manual also contains a plethora of ancillary information including thorough sections on pediatrics, geriatrics, injuries, bites and stings, patient care, alternative medicine, dietary supplements, rehabilitation, normal test values and even legal issues such as advance directives. Yup, it’s pricey, but it’s always proved to be valuable to me.
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Reviewed in the United States on June 15, 2018

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