MYH7B (Myosin Heavy Chain 7B, Cardiac Muscle Beta) ELISA Kit (Small Sample)

MYH7B (Myosin Heavy Chain 7B, Cardiac Muscle Beta) ELISA Kit (Small Sample)The MYH7B (Myosin Heavy Chain 7B, Cardiac Muscle Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MYH7B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MYH7B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB

The MYH7B (Myosin Heavy Chain 7B, Cardiac Muscle Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYH7B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYH7B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYH7B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYH7B in the samples is then determined by comparing the OD of the samples to the standard curve.