SKU: 10841198109

Human LAG3 (Lymphocyte activation gene 3 protein) ELISA Kit

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Ships within 48 hours · Estimated delivery Jul 20 - Jul 25

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Description

Human LAG3 (Lymphocyte activation gene 3 protein) ELISA KitThe Human LAG3 (Lymphocyte activation gene 3 protein) ELISA Kit measures LAG3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LAG3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human LAG3 (Lymphocyte activation gene 3 protein) ELISA Kit measures LAG3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LAG3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with LAG3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of LAG3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 10841198109

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4.3 ★★★★★
Based on 5 reviews
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Justmy2centsworth
Natrona Heights, US
★★★★★ 4
Had a 6mo. old teething (English Bulldog) & this has entertained her for 9 mo.!
Size: Medium, Size: Medium
A chew toy lasting over 6 mo. is generally very good, for this breed & this brand! I kept it in her cage at night, so she wouldn't get board & start chewing on her paws. BC I felt it was a safe toy to use in this manner. "It was safe for my teething Bulldog." "Much, much better than other chew toys from this brand!" Others such as the Orange Pretzel they absolutely love! However, they have always splintered into very sharp edges of plastic, which cause the dogs mouth's to bleed (pokes their gums) & then NEED THROWN AWAY! This Key Chain Chew Toy was "really good" compared to other Nylabone brand chew toys, I have purchased! My girls mouth did not bleed from chewing on it:) "So, I am very pleased with this teething ring!" & would buy it again! Notice how the plastic splinters on the ends? Once the toy gets to his point I feel it is definitely time to be replaced! I would recommend this chew toy for a teething med. sized dog! The one I purchased was fairly large. & stayed together safely:) This is the only Nylabone Chew Toy I would ever, leave in a crate at night, thus far! Good Job Nylabone!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 5, 2022
K
Verified Purchase
Kristin H.
Chelsea, US
★★★★★ 5
Great teething aid
Size: Small
Puppy loves these. I’ve repurchased a couple of times for my teething pup.
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Reviewed in the United States on March 1, 2026
A
Verified Purchase
ANGELA C
Massapequa, US
★★★★★ 5
My dogs love their "keys".
Size: X-Small
My dog loves this. The only negative is that he does chew through it and I have to pick up the pieces or hope that he's not eating it.
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Reviewed in the United States on January 29, 2026
C
Verified Purchase
Cora Jane
Battle Creek, US
★★★★★ 3
Not long lasting for a chewer!
Size: Medium
These are massive! I have a golden retriever puppy and she chewed through the yellow ring very quickly. They keys are much more durable.
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Reviewed in the United States on March 25, 2026
P
Verified Purchase
Pudgy
Fort Morgan, US
★★★★★ 5
Size small. Perfect for little guys
Size: X-Small
These are my pups favorite chewy. He’s 4 months 5lbs. I was having issues with chew toys being too big. I got the small. They are perfect!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 7, 2026

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